Kinetic Data of RNA-Protein Interaction

Protein Information

Protein NameUnr
Protein SourceHomo sapiens
UniProt O75534
SequenceMSFDPNLLHNNGHNGYPNGT...
Mutationwild
Residue Rangefull-length
Nucleic Acid Sequence and Gene Databases ▼
EMBLAF077054, AB020692, AK290146, CR749378, CR749431, AL096773, CH471122, CH471122, BC032446, AF070542
EnsemblENST00000261443.9, ENST00000339438.10, ENST00000358528.9, ENST00000369530.5, ENST00000438362.7, ENST00000534699.5, ENST00000610726.5, ENST00000684913.1, ENST00000686025.1, ENST00000686235.1, ENST00000688211.1, ENST00000689217.1, ENST00000689732.1, ENST00000689989.1, ENST00000692719.1, ENST00000693467.1
RefSeqNP_001007554.1, NP_001123995.1, NP_001229820.1, NP_001229821.1, NP_001229822.1, NP_009089.4
Gene ID7812
Domain and Functional Classification Databases ▼
PfamPF00313, PF23456, PF12901
SMART SM00357
InterProIPR011129, IPR019844, IPR056400, IPR002059, IPR012340, IPR024642
Protein-Protein Interaction Databases ▼
DIPNone
BioGRID113583
STRING9606.ENSP00000481762
Gene Ontology and Pathway Databases ▼
GOGO:0070937, GO:0010494, GO:0005829, GO:0005794, GO:0106002, GO:0000932, GO:0005886, GO:0106222, GO:0003729, GO:1905172, GO:0003723, GO:0035613, GO:0070934, GO:0075522, GO:0008584, GO:1900152, GO:0070966, GO:2000767, GO:0045727, GO:0006446, GO:0034063
ReactomeNone
KEGGhsa:7812
Disease and Drug Databases ▼
DrugBankNone
MalacardsPA142672072
DisGeNET7812
TTDNone

Reference Information

TitleRNA binding specificity of Unr, a protein with five cold shock domains.
JournalNucleic acids research (1999)
DOI10.1093/nar/27.8.1926
PubMed10101203
LocationTable 1

RNA Information

RNA Nameselect 98
RNA SourceSynthetic nucleic acid
Type of RNAUnclassified RNA
Sequence of chain 1
UGAUGAAGUAAA
Sequence of chain 2
Mutationwild
RNA Databases ▼
PDBe linkNone
RNACentralNone
ENANone
MalacardsNone
GeneCardsNone

Binding Information

Kd (Wild,M)2.10E-08
Kd (Mutation,M)
Ka (Wild,M-1)4.76E+07
Ka (Mutation,M-1)
kon (M-1s-1)
koff (s-1)
pH8
Temperature303
Buffer25 μg/ml BSA, 10 ng of tRNA, 1 mM beta-mercaptoethanol and 1 mM PMSF, in TNG buffer
MethodFilter binding assay
Addition

Structure Information

Protein Structure
Very high (pLDDT > 90)
High (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (50 > pLDDT)
Unr (O75534): full-length
Experiment Structures
PDB ID Chain Resolution(Å) Range
None
AlphaFold-predicted Structure
Model ID Avg pLDDT Range
AF-O75534-F1 79.56 1-798

Download
Full-length protein predicted by AlphaFold Download
RNA Structure
Very high (pLDDT > 90)
High (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (50 > pLDDT)
magnifier
Download

Secondary RNA structure predicted by RNAstructure

Tertiary RNA structure predicted by RhoFold+ Download
Complex Structure
Click to predict 3D structure in AlphaFold3

Related Entries

ID Protein Name Uniprot RNA Name koff (s-1) kon(M -1s-1) Kd (M)
183 Unr O75534 select 85 None None 1.10E-08
184 Unr O75534 select 88 None None 1.00E-08
185 Unr O75534 select 76 None None 1.00E-08
187 Unr O75534 select 59 None None 1.30E-08
188 Unr O75534 select 10b None None 1.20E-08
189 Unr O75534 select 36b None None 1.00E-08
190 Unr O75534 select 23b None None 1.20E-08
191 Unr O75534 select 28b None None 1.60E-08
192 Unr O75534 select 29b None None 1.80E-08
193 Unr O75534 select 77 None None 1.00E-08
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